Giovanni Mandarano, Jyoti Arora, Dominic Wall, Simon Harrison, Noelene Bergen, Dodie Pouniotis and Peter Eu
The utilisation of magnetic resonance imaging (MRI) to noninvasively monitor cells using superparamagnetic iron oxide nanoparticles (SPIONs) as MRI contrast agents has been one of the major research focuses over the past several years. The ability to use MRI to image longitudinally offers an invaluable opportunity to track the migration, persistence and distribution of cell based therapeutics in vivo in humans.
Current approaches for cell labelling have focused on using commercial SPIONs with varying success. Therefore, there is a clinical need to develop SPIONs that are non-toxic, with appropriate size and high MRI contrast. We have utilised SPIONs with a silica coating to investigate the labelling ability and contrast enhancement of CD45+ and CD34+ expressing cells.